期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 59, 期 20, 页码 10839-10847出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf2023325
关键词
tea; amino adds; high-performance liquid chromatography with fluorescence detection (HPLC-FLD); second-order calibration; alternating penalty trilinear decomposition algorithm (APTLD)
资金
- Ministry of Science and Technology of China [2004DEA71180]
- Science and Technology Department of Sichuan Province [2006G11-003, 2009NZ0015]
In this paper, a novel application of alternating penalty trilinear decomposition (APTLD) for high-performance liquid chromatography with fluorescence detection (HPLC-FLD) has been developed to simultaneously determine the contents of free amino adds in tea. Although the spectra of amino add derivatives were similar and a large number of water-soluble compounds are coextracted, APTLD could predict the accurate concentrations together with reasonable resolution of chromatographic and spectral profiles for the amino acids of interest owing to its second-order advantage. An additional advantage of the proposed method is lower cost than traditional methods. The results indicate that it is an attractive alternative strategy for the routine resolution and quantification of amino acids in the presence of unknown interferences or when complete separation is not easily achieved.
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