期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 59, 期 18, 页码 9942-9949出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf202501e
关键词
high-level expression; HSP terminator; miraculin; transgenic tomato plants; CaMV 35S promoter
资金
- Ministry of Economy, Trade, and Industry of Japan
- MEXT, Japan [TOMJPF00001]
- Grants-in-Aid for Scientific Research [23880001] Funding Source: KAKEN
High-level accumulation of the target recombinant protein is a significant issue in heterologous protein expression using transgenic plants. Miraculin, a taste modifying protein, was accumulated in transgenic tomatoes using an expression cassette in which the miraculin gene was expressed by the cauliflower mosaic virus (CaMV) 35S promoter and the heat shock protein (HSP) terminator (MIR-HSP). The HSP terminator was derived from heat shock protein 18.2 in Arabidopsis thaliana. Using this HSP-containing cassette, the miraculin concentration in To transgenic tomato lines was 1.4-13.9% of the total soluble protein (TSP), and that in the T-1 transgenic tomato line homozygous for the miraculin gene reached 17.1% of the TSP. The accumulation level of the target protein was comparable to levels observed with chloroplast transformation. The high-level accumulation of miraculin in To transgenic tomato lines achieved by the HSP terminator was maintained in the successive T-1 generation, demonstrating the genetic stability of this accumulation system.
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