期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 59, 期 8, 页码 4164-4170出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf1046909
关键词
t,t-Conjugated linoleic acid (CLA); human mammary epithelial cell line MCF-10A; 12-O-tetradecanoylphorbol-13acetate (TPA); gap junctional intercellular communication (GJIC); connexin43 (Cx43); reactive oxygen species (ROS)
资金
- BK21 Program
- Ministry of Education, Science, and Technology, Republic of Korea [2009-0075483]
- National Research Foundation of Korea [2009-0075483] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The anti-tumor promotional effects of t9,t11-conjugated linoleic acid (t9,t11-CLA) and t10,t12-CLA were evaluated on the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inhibition of gap junctional intercellular communication (GJIC) in the human mammary epithelial cell line MCF-10A. The results were compared to those obtained from c9,t11-CLA, which is a more effective anti-tumor promoter on TPA-induced GJIC inhibition in MCF-10A cells than t10,c12-CLA. Cells were treated with 20 mu M t9,t11-CLA, t10,t12-CLA, or c9,t11-CLA for 24 h followed by 60 nM TPA for 1 h. Both t9,t11-CLA and t10,t12-CLA equally protected MCF-10A cells from TPA-induced inhibition of GJIC with inferior efficacy to c9,t11-CLA. The protection was due to the ameliorated phosphorylation of connexin43 via suppression of extracellular signal-regulated Kinases (ERK1/2) activation. Suppression of TPA-induced reactive oxygen species (ROS) generation by t9,t11-CLA and t10,t12-CLA was less effective, relative to c9,t11-CLA. The results suggest that the anti-promotional activities of t9,t11-CLA and t10,t12-CLA are equal but less potent than c9, t11-CLA in TPA-treated MCF-10A cells. The activity might be mediated by the attenuation of ROS production in MCF-10A cells by preventing the downregulation of GJIC during the cancer promotion stage.
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