期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 59, 期 21, 页码 11794-11802出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf203060s
关键词
trypsin; procyanidin; carbohydrates; STD-NMR; fluorescence quenching
资金
- FCT (Fundacao para a Ciencia e Tecnologia) of Portugal
- NMR Lab at CEMUP (Centro de Materials da Universidade do Porto) [REDE/1517/RMN/2005]
- FCT [SFRH/BD/38814/2007]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/38814/2007] Funding Source: FCT
The biological properties of procyanidins, in particular their inhibition of digestive enzymes, have received much attention in the past few years. Dietary carbohydrates are an environmental factor that is known to affect the interaction of procyanidins with proteins. This work aimed at understanding the effect of ionic food carbohydrates (polygalacturonic acid, arabic gum, pectin, and xanthan gum) on the interaction between procyanidins and trypsin. Physical-chemical techniques such as saturation transfer difference-NMR (STD-NMR) spectroscopy, fluorescence quenching, and nephelometry were used to evaluate the interaction process. Using STD-NMR, it was possible to identify the binding of procyanidin B3 to trypsin. The tested carbohydrates prevented the association of procyanidin B3 and trypsin by a competition mechanism in which the ionic character of carbohydrates and their ability to encapsulate procyanidins seem crucial leading to a reduction in STD signal and light scattering and to a recovery of the proteins intrinsic fluorescence. On the basis of these results, it was possible to grade the carbohydrates in their aggregation inhibition ability: XG > PA > AG >> PC. These effects may be relevant since the coingestion of procyanidins and ionic carbohydrates are frequent and furthermore since these might negatively affect the antinutritional properties ascribed to procyanidins in the past.
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