期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 58, 期 20, 页码 11097-11104出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf1024533
关键词
Rice (Oryza sativa L.); high-amylose starch; C-type starch; endosperm development; starch granule formation
资金
- National Natural Science Foundation of China [30828021, 30971754, 31071342]
- Ministry of Science and Technology [2009ZX08011-003B, 2008ZX08009-003]
- Natural Science Foundation of Jiangsu Province [BK2009186]
- China Postdoctoral Science Foundation [20090451252]
Cereal starch granules with high-amylose and resistant starch (RS) always show irregular morphology and special crystalline structure, but their formation during grain development is not yet clear. In our previous studies, we had generated a transgenic rice line (TRS) enriched with amylose and RS, which contained semi-compound starch showing a C-type crystalline structure. In this study, the formation of semi-compound C-type starch granule during TRS endosperm development was carefully investigated with light, scanning electron, and transmission electron microscopes and X-ray powder diffraction. The results showed that the TRS starch subgranules, each with a central hilum, were individually initiated in amyloplast and showed an A-type crystal at the early stage of starch granule development, which was similar to that in its wild type. However, with the endosperm development, the amylose content in TRS endosperm starch increased and the B-type starch crystal was deposited in the periphery of subgranules; then, the adjacent subgranules fused together and finally formed a continuous outer layer band surrounding the entire circumference of the starch granule. Accordingly, a mechanistic model for the formation of semi-compound C-type starch granules is proposed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据