The Effect of Monascus Secondary Polyketide Metabolites, Monascin and Ankaflavin, on Adipogenesis and Lipolysis Activity in 3T3-L1

The aim of the present work is to investigate the effects of Monascus secondary metabolites, monascin (MS) and ankaflavin (AK), on cell proliferation, adipogenesis, lipolysis and heparin-releasable lipoprotein lipase (HR-LPL) in 3T3-L1 preadipocyte. MS and AK inhibit the proliferation of 3T3-L1 cells in a dose-dependent fashion. At 8 mu g/mL concentration MS inhibits proliferation for 80.5% after 48 h, whereas the value for AK is 69.2%. Adipogenesis is inhibited by MS and AK without dose-dependency. Triglyceride is decreased 37.1% and 41.1% respectively by treating 0.125 mu g/mL MS and AK. Adipocyte-specific transcription factors peroxisome proliferator-activated receptor gamma (PPAR gamma), CCAAT/enhancer binding protein beta (C/EBP beta), C/EBP delta and C/EBP alpha mRNA levels are measured by real-time polymerase chain reaction. The expression of the four transcriptional factors analyzed (PPAR gamma, C/EBP beta, C/EBP delta and C/EBP alpha) is reduced at the initial and the middle period. At the later period, there is no effect on the expression of PPAR gamma and C/EBP alpha by treating MS and AK. Furthermore, both MS and AK increase basal lipolysis of mature adipocytes by 113.2% and 278.3% upregulation, respectively. And both MS and AK reduce the activity of HR-LPL, by 45.3% and 58.1% reduction, respectively. This study reveals for the first time that Monascus secondary metabolites, MS and AK, can prevent the differentiatioin of preadipocyte and stimulate basal lipolysis of mature adipocytes, avoiding the accumulation of lipid.