期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 58, 期 24, 页码 12993-12998出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf103723k
关键词
Tyrosinase; cardol triene; inhibitory kinetics; competitive inhibition
资金
- Ministry of Science and Technology [2010CB530002]
- Natural Science Foundation of China [31071611]
- Science and Technology Foundation of Fujian Province [2010N5013]
Cardol triene was first purified from cashew (Anacardium occidentale L.) nut shell liquid and identified by gas chromatography coupled to mass spectroscopy and nuclear magnetic resonance. The effects of this compound on the activity of mushroom tyrosinase were studied. The results of the kinetic study showed that cardol triene was a potent irreversible competitive inhibitor and the inactivation was of the complexing type. Two molecules of cardol triene could bind to one molecule of tyrosinase and lead to the complete loss of its catalytic activity. The microscopic rate constants were determined for the reaction of cardol triene with the enzyme. The anti-tyrosinase kinetic research of this study provides a comprehensive understanding of inhibitory mechanisms of resorcinolic lipids and is beneficial for the future design of novel tyrosinase inhibitors.
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