期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 58, 期 12, 页码 7383-7388出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf100385m
关键词
Rice (Oryza sativa L.); high-amylose resistant starch granule; C-type starch; X-ray powder diffraction; solid-state nuclear magnetic resonance
资金
- National Natural Science Foundation of China [30828021, 30300215]
- Ministry of Science and Technology [2006AA10A102, 2009ZX08011-003B]
- Government of Jiangsu Province [BK2009186, 06KJA21018]
- China Postdoctoral Science Foundation [20090451252]
High-amylose starch is a source of resistant starch (RS) which has a great benefit on human health. A transgenic rice line (TRS) enriched amylose and AS had been developed by antisense RNA inhibition of starch branching enzymes. In this study, the native starch granules were isolated from TRS grains as well as the wild type, and their crystalline type was carefully investigated before and after acid hydrolysis. In high-amylose TRS rice, the C-type starch, which might result from the combination of both A-type and B-type starch, was observed and subsequently confirmed by multiple physical techniques, including X-ray powder diffraction, solid-state nuclear magnetic resonance, and Fourier transform infrared. Moreover, the change of starch crystalline structure from C- to B-type during acid hydrolysis was also observed in this RS-rich rice. These data could add to our understanding of not only the polymorph structure of cereal starch but also why high-amylose starch is more resistant to digestion.
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