期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 58, 期 2, 页码 1333-1341出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf9032443
关键词
Macrophage CD36; oxidized LDL; PPAR gamma; quercitrin; VEGF
资金
- Ministry of Knowledge Economy through Technological Development Project for Regional Strategic Planning, Korea
- Regional Research Universities Program/Medical & Bio-Materials Research Center, the Korean Government (MEST)
Oxidized LDL (oxLDL) has been implicated in the pathogenesis of atherosclerosis accompanying lipid-laden cell appearance, inflammatory responses, and vascular dysfunction. This study examined the potentials of polyphenol quercitrin to inhibit oxLDL induction of scavenger receptor A (SR-A) and CD36 involving activation of peroxisome proliferator-activated receptor gamma (PPAR gamma). J774A1 murine macrophages were Cultured with 10 mu g/mL Cu2+-oxLDL for various times in the presence of 1 - 10 mu mol/L quercitrin. Cu2+-oxLDL at the given concentration facilitated macrophage proliferation and enhanced oxLDL uptake. Quercitrin dampened oxLDL uptake and lipid accumulation elevated in macrophages exposed to oxLDL. Western blot analysis revealed that 10 mu g/mL oxLDL upregulated expression of SR-A and CD36, which was rapidly abolished at the transcriptional levels by 10 mu mol/L quercitrin within 4 h. Quercitrin diminished production of proinflammatory and proatherogenic vascular endothelial growth factor that augmented through the oxLDL binding to CD36. Similarly, quercitrin repressed expression of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1 involved in monocyte trafficking and macropahage migration. In addition, quercitrin attenuated oxLDL-induced transcriptional activation of PPAR gamma leading to CD36 induction. Furthermore, quercitrin alleviated macrophage uptake of oxLDL through interfering with PKC-PPAR signaling cascades. These results demonstrate that quercitrin blocked p LDL uptake, cholesterol influx and lipid-laden foam cell formation through inhibiting induction of R and VEGF linked to PKC alpha-PPAR gamma-responsive pathways. Therefore, quercitrin may be an antiatherogenic agent blocking foam cell formation pertaining to induction of SR and VEGF.
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