期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 57, 期 10, 页码 4035-4041出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf900075h
关键词
Zearalenone; immmunochromatographic assay; validation; gold nanoparticle; corn
资金
- KOSEF/MOST [R15-2003-012-01001-0]
- Ministry of Education, Science and Technology, Korea
- National Research Foundation of Korea [R15-2003-012-01001-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A monoclonal antibody (mAb)-based gold nanoparticle immunochromatographic assay (ICG) for zearalenone detection was developed, optimized, and validated. The detection limits of ICG optimized with appropriate amounts of zearalenone-bovine serum albumin and gold nanoparticle-mAb to zearalenone were 2.5 ng/mL and 30 mu g/kg for the standard solution and spike sample, respectively, and a weak cross-reaction for alpha-zearalenol and beta-zearalenol was observed. The assay required only 15 min to obtain results and one step to perform the assay. In validation, the results obtained from spiked corn (10, 20, 30, 50, and 100 mu g/kg) and naturally contaminated corn samples by the ICG were in good agreement with those obtained by direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and high-performance liquid chromatography (HPLC). Therefore, the results obtained in this study could be used as basic research for the development of zearalenone-ICG, and the ICG developed could be a useful on-site screening tool for the rapid detection of zearalenone in corn without special instrumentation.
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