期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 57, 期 15, 页码 6712-6719出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf9009894
关键词
Campylotropis hirtella; geranylated flavonoids; Leguminosaea; immunosuppressants
In an effort to identify new immunosuppressive agents from natural sources, 12 new geranylated flavonoids, 5,7,4'-trihydroxy-3'-[7-hydroxy-3,7-dimethyl-2(E)-octenyl]isoflavone (1), a racemate of 5,7,2',4'-tetrahydroxy-3'-[7-hydroxy-3,7-dimethyl-2(E)-octenyl]isoflavanone (2), 2 ''(S)-5,7-dihydroxy[2 ''-methyl-2 ''-(4-methyl-3-pentenyl)pyrano]-5 '',6 '':3',4 ''-isoflavone (3), (2 '' S,3 '' R,4 '' S)-5,7,3 '',4-tetrahydroxy[2 ''-methyl-2 ''-(4-methyl-3-pentenyI)pyrano]-5 '',6 '':3',4'-isoflavone (4), a racemate of 3'-geranyl5,7,2',4'-tetrahydroxyisoflavanone (5), a racemate of 3' -geranyl-4'-methoxy-5,7,2'-trihydroxyisoflavanone (6), 3'-geranyl-5,7,4',5'-tetrahydroxyisoflavone (8), 3'-geranyl-5,7,2',5'-tetrahydroxyisoflavone (9), 3'-geranyl-4'-methoxy-5,7,2'-trihydroxyisoflavone (10), 2(R),3(R)-3'-geranyl-2,3-trans-5,7,4-trihydroxyflavonol (12), (2R,3R)-6-methyl-3'-geranyl-2,3-trans-5,7,4'-trihydroxytlavonol (13), and 5,7-dihydroxy-4'-O-geranylisoflavone (14), were isolated from the roots of Campylotropis hirtella (Franch.) Schindl. together with three previously described flavonoids. Their structures were elucidated by spectroscopic measurements, including two-dimensional nuclear magnetic resonance (NMR) techniques. The immunosuppressive effects of these compounds were assessed using mitogen-induced splenocyte proliferation, and the cytotoxicity of the compounds was also examined. The IC50 values of the compounds were found to be in the range of 1.49-61.23 mu M for T lymphocyte suppression and 1.16-73.07 mu M for B lymphocyte suppression. An analysis of their structure-activity relationships revealed that an isoflavonoid carbon skeleton with a C10 substituent at the C31 position was necessary for the activity. As many of the compounds exhibited good immunosuppressive activities, they may be promising as novel immunosuppressive agents.
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