期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 56, 期 16, 页码 6873-6880出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf801332r
关键词
honey; PCA; hierarchical PLS-DA; H-1 and C-13 NMR; geographical and botanical characterization
The importance of geographical origin determination is an increasing and pressing requirement for all foods. Honey is one of the largest studied foods due to its nutritional and medicinal properties in a correct diet. In this paper, a total of 41 honey samples (polyfloral and acacia) from different countries have been analyzed in terms of H-1 NMR spectroscopy coupled with multivariate statistical methods. Unsupervised principal component analysis resulted as an efficient tool in distinguishing H-1 NMR spectra of polyfloral and acacia honey samples and for geographical characterization of the latter ones. Hierarchical projection to latent structures discriminant analysis was successfully applied for the discrimination among polyfloral honey samples of different geographical origins. C-13 NMR spectroscopy was applied to honey samples with the aim to investigate possible sugar isoforms differentiation. Our preliminary data indicated a different isoforms ratio between beta FP and beta FF only for polyfloral Argentinean samples, while Hungarian samples showed resonance shifts for some carbons of alpha FF, beta FP, beta FF, and (alpha GP isoforms for both varieties. These data confirmed the potentiality of C-13 spectroscopy in food characterization, especially in sugar-based foods.
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