期刊
JAPANESE JOURNAL OF INFECTIOUS DISEASES
卷 67, 期 4, 页码 307-310出版社
NATL INST INFECTIOUS DISEASES
DOI: 10.7883/yoken.67.307
关键词
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资金
- HIV/AIDS [H24-AIDS-Wakate-002]
- Research for Intractable Infectious Diseases in Organ Transplant Recipients [H21-Shinko-Ippan-009]
- Ministry of Health, Labour and Welfare of Japan [H22-Nanchi-Ippan-013]
Quantitative PCR testing for JC virus (JCV) DNA in the cerebrospinal fluid (CSF) is one of the diagnostic standards for progressive multifocal leukoencephalopathy (PML). The present study was conducted to examine its reliability using CSF specimens that had been preserved with guanidine lysis buffers in commercial nucleic acid extraction kits under different conditions. When CSFs were mixed with guanidine buffers, JCV DNA levels were not statistically reduced even after storage for 1 month at room temperature or for 3 months at -80 degrees C, compared with the control samples. In addition, the JCV DNA level was not decreased in a mixture of CSF and guanidine thiocyanate buffer incubated for 3 days at 56 C. These data suggest that CSF specimens mixed with commercial guanidine buffers can be stored without refrigeration, more safely handled, and directly subjected to JCV DNA testing for PML.
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