期刊
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 8
卷 8, 期 -, 页码 463-483出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev-anchem-071114-040240
关键词
glycoprotein; mass spectrometry; glycopeptide; liquid chromatography; tandem MS; software
资金
- National Institutes of Health [1R01AI094797]
- American Chemical Society Division of Analytical Chemistry Graduate Fellowship - Eastman Chemical Company
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI094797] Funding Source: NIH RePORTER
Glycosylation on proteins adds complexity and versatility to these biologically vital macromolecules. To unveil the structure-function relationship of glycoproteins, glycopeptide-centric analysis using mass spectrometry (MS) has become a method of choice because the glycan is preserved on the glycosylation site and site-specific glycosylation profiles of proteins can be readily determined. However, glycopeptide analysis is still challenging given that glycopeptides are usually low in abundance and relatively difficult to detect and the resulting data require expertise to analyze. Viewing the urgent need to address these challenges, emerging methods and techniques are being developed with the goal of analyzing glycopeptides in a sensitive, comprehensive, and high-throughput manner. In this review, we discuss recent advances in glycoprotein and glycopeptide analysis, with topics covering sample preparation, analytical separation, MS and tandem MS techniques, as well as data interpretation and automation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据