期刊
MOLECULES
卷 20, 期 2, 页码 3206-3220出版社
MDPI
DOI: 10.3390/molecules20023206
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资金
- Universiti Kebangsaan Malaysia (UKM) grant [GUP-2012-001]
The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E-2 (PGE(2)) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE(2) production with IC50 values of 1.05, 1.54, and 2.59 mu M, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 mu M). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids.
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