期刊
MOLECULES
卷 21, 期 1, 页码 -出版社
MDPI AG
DOI: 10.3390/molecules21010027
关键词
monoclonal antibody; colloidal gold; fluorescent microsphere; lateral-flow immuno-chromatographic assay; T-2 toxin
资金
- National Natural Science of China-Guangdong Joint Fund Project [U1301214]
- Special Fund for Agro-scientific Research in the Public Interest [201203040]
A new highly specific and sensitive monoclonal antibody (MAb) to T-2 toxin (T-2) was produced, providing an IC50 value of 1.02 ng/mL and negligible cross-reactivity (CR) to other related mycotoxins. Based on the new MAb, a lateral-flow immunochromatographic assay (LFIA) using colloidal gold (CG) and fluorescent microspheres (FMs) as labels was proposed for T-2. Under the optimized conditions, in rapid qualitative assay, the cut-off values of the CG-LFIA were 400 g/kg in rice and 50 g/L in fresh milk, and the cut-off values of the FMs-LFIA were 100 g/kg in both rice and chicken feed. For the quantitative assay with the FMs-LFIA, the limit of detection (LOD) were 0.23 g/kg and 0.41 g/kg in rice and chicken feed, respectively, and the average recoveries ranged from 80.2% to 100.8% with the coefficient of variation (CV) below 10.8%. In addition, we found that the CG-LFIA could tolerate the matrix effect of fresh milk better than the FMs-LFIA, while the FMs-LFIA could tolerate the matrix effect of chicken feed better than CG-LFIA under the same experimental conditions. These results provide a certain reference for the selection of appropriate labels to establish a rapid LFIA in various biological samples.
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