期刊
IUBMB LIFE
卷 64, 期 5, 页码 450-454出版社
WILEY-BLACKWELL
DOI: 10.1002/iub.567
关键词
human serum albumin; antioxidant activity; reactive oxygen species; nitric oxide; cysteine; methionine
资金
- Grants-in-Aid for Scientific Research [22790162, 23659088] Funding Source: KAKEN
The importance of cysteine (Cys) and methionine (Met) residues for the antioxidant activity of human serum albumin (HSA) was investigated using recombinant HSA mutants, in which Cys34 and/or the six Met residues had been mutated to Ala. The scavenging activities of the mutants against five reactive oxygen and nitrogen species were evaluated by a chemiluminescence assay, electron paramagnetic resonance spectroscopy, or a HPLC-flow reactor assay. Our results showed that the contributions of Cys34 and the Met residues to the antioxidant activity of HSA were 61% and 29% against O2 -, 68% and 61% against H2O2, 38% and 6% against HO, 36% and 13% against HOCl, and 51% and 1% against NO, respectively. Thus, the findings propose in a direct way that Cys34 plays a more important role than the Met residues in the antioxidant activity of HSA. (c) 2012 IUBMB Life, 2012
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