期刊
IUBMB LIFE
卷 63, 期 12, 页码 1075-1080出版社
WILEY
DOI: 10.1002/iub.530
关键词
Bioreporters; chromosomal reporters; gene amplification; gene duplication; gene transfer; genetic engineering; hypersusceptibility; metabolic engineering; transformation
资金
- National Science Foundation [MCB 0920893, DBI 0905829]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [0905829] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0920893] Funding Source: National Science Foundation
For more than 25 years, Acinetobacter baylyi ADP1 has been used in molecular biology studies that address a broad range of questions. Recently, the rapid accumulation of data from DNA sequencing, gene expression, protein structure, and other high-throughput methodology has increased the ability to tackle complex topics using sophisticated approaches to metabolic and genetic engineering. While the genetic malleability of ADP1 makes it an ideal organism for such investigations, A. baylyi ADP1 has yet to become a common choice for bacterial studies. This review describes examples of ADP1-based studies that exploit its highly efficient system for natural transformation and chromosomal incorporation of exogenous DNA. These studies focus on a wide array of problems, including gene duplication and amplification, horizontal gene transfer, bioreporters, and metabolic reconstruction. Interesting results in these diverse areas highlight the utility of using A. baylyi in laboratory and industrial settings. (C) 2011 IUBMB IUBMB Life, 63(12): 1075-1080, 2011
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