期刊
IUBMB LIFE
卷 63, 期 5, 页码 355-362出版社
WILEY-BLACKWELL
DOI: 10.1002/iub.470
关键词
heme proteins; hemoglobin; oxygen metabolism; protein function
资金
- Italian Ministero dell'Istruzione, dell'Universita e della Ricerca [2004052135, 2008BFJ34R]
- Spanish Ministerio de Innovacion y Ciencia [SAF2008-05595, IT2009-0010]
- Generalitat de Catalunya [SGR2009-294, XRQTC]
- EU
Nonsymbiotic hemoglobins AHb1 and AHb2 discovered in Arabidopsis thaliana are likely to carry out distinct physiological roles, in consideration of their differences in sequence, structure, expression pattern, and tissue localization. Despite a relatively fast autoxidation in the presence of O(2), we were able to collect O(2)-binding curves for AHb2 in the presence of a reduction enzymatic system. AHb2 binds O(2) noncooperatively with a p50 of 0.021 +/- 0.003 Torr, a value consistent with a recently proposed role in O(2) transport. The analysis of the internal cavities derived from the structures sampled in molecular dynamics simulations confirms strong differences with AHb1, proposed to work as a NO deoxygenase in vivo. Overall, our results are consistent with a role for AHb2 as an oxygen carrier, as recently proposed on the basis of experiments on AHb2-overexpressing mutants of A. thaliana. (C) 2011 IUBMB IUBMB Life, 63(5): 355-362, 2011
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