期刊
IUBMB LIFE
卷 61, 期 11, 页码 1029-1042出版社
WILEY
DOI: 10.1002/iub.256
关键词
live cell imaging; coral fluorescent proteins; GFP; RFP; monomeric red fluorescent protein; far red fluorescent protein; photoconversion; photoactivation; super resolution microscopy
资金
- Deutsche Forschungsgerneinschaft (DFG) [Wi-1990/2-1, Ni 291/9]
- State of Baden-Wurttemberg
- Center for Functional Nanostructures (CFN)
- Fonds der Chemischen Industrie to GUN
- Landestiftung Baden-Wurttemberg
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of. the glowing probes. (C) 2009 IUBMB IUBMB Life, 61(11): 1029-1042, 2009
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