期刊
ISRAEL JOURNAL OF PLANT SCIENCES
卷 56, 期 1-2, 页码 37-44出版社
BRILL
DOI: 10.1560/IJPS.56.1-2.37
关键词
Nannochloropsis; inorganic carbon; ribulose-1,5-bisphosphate carboxylase/oxygenase; RubisCO; specificity factor; membrane inlet mass spectrometry
The marine unicellular alga Nannochloropsis sp. (Eustigmatophyceae) exhibits high apparent affinity for extracellular inorganic carbon (Ci) despite the fact that its ability to accumulate Ci within the cells is relatively low. Kinetic investigation of carboxylation enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), isolated from Nannochloropsis suggests that the latter discrepancy call be accounted for by the high affinity of RubisCO for CO2. A K-m(CO2) of 7-10 mu M was obtained both by a standard radiolabeling approach and by novel methodology using membrane inlet mass spectrometry. The latter allows precise determination of the changes in the concentrations of dissolved CO2 and O-2 as the reaction proceeds. The kinetic parameters of the oxygenase reaction, deduced from measurements of oxygen level, indicated a high K-m (O-2) (about 1 mM) and high V-max (3.9 mu mol O-2 min(-1) mg(-1) protein) values, compared to those observed in green algae. Thus, despite Nannochloropsis RubisCO's low K.(CO2), all unusually low specificity factor of 27 was calculated, lower than observed in cyanobacteria and close to values found in anaerobic organisms. We proposed that the elevated CO2 level within the cells, indicated by massive net efflux of CO2 during steady state photosynthesis, is essential for its growth under the high O-2 concentrations prevailing in the environment.
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