期刊
ISRAEL JOURNAL OF CHEMISTRY
卷 51, 期 8-9, 页码 862-867出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ijch.201100128
关键词
chemical ligation; peptides; protein engineering; protein modifications; selenocysteine
Of the many approaches proposed to generalize the native chemical ligation approach for protein synthesis, the simple procedure of global desulfurization of peptide thiols has become the most widely adopted. In this review, the development of the native ligationdesulfurization strategy is described, focusing on the conversion of Cys to Ala following ligation at N-terminal Cys residues. Subsequent variations on this theme have broadened the scope to other natural amino acids including Phe, Leu, Val, and Lys, and even non-native peptide linkages such as isopeptide bonds on lysine side chains. Using insights from both selenocysteinepeptide side reactions and radical initiated desulfurization procedures, a new method for the selective deselenization of peptides containing both selenocysteine and cysteine residues has been developed. Together, these approaches represent a robust and flexible methodology for the synthesis of complex polypeptides without the use of protecting groups.
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