期刊
ISME JOURNAL
卷 12, 期 12, 页码 3043-3045出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41396-018-0233-7
关键词
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资金
- National Science Foundation, division of solar programs [1142096]
- NSF [DEB-1241094]
- Department of Energy's Biological Systems Science Division, Program in Genomic Science [DE-SC0010579, DE-SC0016207]
- IGERT Fellowship
- Directorate For Geosciences
- Office of Polar Programs (OPP) [1142096] Funding Source: National Science Foundation
- U.S. Department of Energy (DOE) [DE-SC0010579, DE-SC0016207] Funding Source: U.S. Department of Energy (DOE)
Most soil bacterial taxa are thought to be dormant, or inactive, yet the extent to which they synthetize new rRNA is poorly understood. We analyzed O-18 composition of RNA extracted from soil incubated with (H2O)-O-18 and used quantitative stable isotope probing to characterize rRNA synthesis among microbial taxa. RNA was not fully labeled with O-18, peaking at a mean of 23.6 +/- 6.8 atom percent excess (APE) O-18 after eight days of incubation, suggesting some ribonucleotides in soil were more than eight days old. Microbial taxa varied in the degree they incorporated O-18 into their rRNA over time and there was no correlation between the APE O-18 of bacterial rRNA and their rRNA to DNA ratios, suggesting that the ratios were not appropriate to measure ribonucleotide synthesis. Our study indicates that, on average, 94% of soil taxa produced new rRNA and therefore were metabolically active.
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