期刊
ISME JOURNAL
卷 9, 期 5, 页码 1076-1092出版社
SPRINGERNATURE
DOI: 10.1038/ismej.2014.198
关键词
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资金
- JCVI internal fund
- Gordon and Betty Moore Foundation [GBMF3828, GMBF521]
- NSF [OCE-0550302, OCE-0550798, OCE-1031064]
- California Current Ecosystem Long Term Ecological Research Program [LTER NSF/OCE-0417616]
- Princeton Center for Bioinorganic Chemistry
- crew of the R/V New Horizon
- US Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-FC02-02ER63453]
- Directorate For Geosciences
- Division Of Ocean Sciences [1031064] Funding Source: National Science Foundation
- Division Of Ocean Sciences
- Directorate For Geosciences [1026607] Funding Source: National Science Foundation
Transitions in community genomic features and biogeochemical processes were examined in surface and subsurface chlorophyll maximum (SCM) microbial communities across a trophic gradient from mesotrophic waters near San Diego, California to the oligotrophic Pacific. Transect end points contrasted in thermocline depth, rates of nitrogen and CO2 uptake, new production and SCM light intensity. Relative to surface waters, bacterial SCM communities displayed greater genetic diversity and enrichment in putative sulfur oxidizers, multiple actinomycetes, low-light-adapted Prochlorococcus and cell-associated viruses. Metagenomic coverage was not correlated with transcriptional activity for several key taxa within Bacteria. Low-light-adapted Prochlorococcus, Synechococcus, and low abundance gamma-proteobacteria enriched in the > 3.0-mu m size fraction contributed disproportionally to global transcription. The abundance of these groups also correlated with community functions, such as primary production or nitrate uptake. In contrast, many of the most abundant bacterioplankton, including SAR11, SAR86, SAR112 and high-light-adapted Prochlorococcus, exhibited low levels of transcriptional activity and were uncorrelated with rate processes. Eukaryotes such as Haptophytes and non-photosynthetic Aveolates were prevalent in surface samples while Mamielles and Pelagophytes dominated the SCM. Metatranscriptomes generated with ribosomal RNA-depleted mRNA (total mRNA) coupled to in vitro polyadenylation compared with polyA-enriched mRNA revealed a trade-off in detection eukaryotic organelle and eukaryotic nuclear origin transcripts, respectively. Gene expression profiles of SCM eukaryote populations, highly similar in sequence identity to the model pelagophyte Pelagomonas sp. CCMP1756, suggest that pelagophytes are responsible for a majority of nitrate assimilation within the SCM.
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