期刊
ISME JOURNAL
卷 6, 期 3, 页码 670-679出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ismej.2011.128
关键词
akinetes; cyanobacteria; fluorescence in situ hybridization; polyphosphate; polyploidy; laser microdissection microscopy
资金
- Gruss Lipper Foundation
- German Ministry of Research and Technology (BMBF) [FKZ 02WT0985, WR803]
- Israel Ministry of Science and Technology (MOST)
Akinetes are dormancy cells commonly found among filamentous cyanobacteria, many of which are toxic and/or nuisance, bloom-forming species. Development of akinetes from vegetative cells is a process that involves morphological and biochemical modifications. Here, we applied a single-cell approach to quantify genome and ribosome content of akinetes and vegetative cells in Aphanizomenon ovalisporum (Cyanobacteria). Vegetative cells of A. ovalisporum were naturally polyploid and contained, on average, eight genome copies per cell. However, the chromosomal content of akinetes increased up to 450 copies, with an average value of 119 genome copies per akinete, 15-fold higher than that in vegetative cells. On the basis of fluorescence in situ hybridization, with a probe targeting 16S rRNA, and detection with confocal laser scanning microscopy, we conclude that ribosomes accumulated in akinetes to a higher level than that found in vegetative cells. We further present evidence that this massive accumulation of nucleic acids in akinetes is likely supported by phosphate supplied from inorganic polyphosphate bodies that were abundantly present in vegetative cells, but notably absent from akinetes. These results are interpreted in the context of cellular investments for proliferation following a long-term dormancy, as the high nucleic acid content would provide the basis for extended survival, rapid resumption of metabolic activity and cell division upon germination. The ISME Journal (2012) 6, 670-679; doi: 10.1038/ismej.2011.128; published online 6 October 2011
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据