期刊
ISME JOURNAL
卷 3, 期 3, 页码 296-304出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ismej.2008.105
关键词
acyl homoserine lactone; marine subtidal biofilm; biofilm formation; bacterial community; dynamics
资金
- RGC [CA04/05.Sc01, HKUST6402/05M]
- Croucher Foundation [CF-CAS03/04.Sc01]
The quorum sensing signals-acyl homoserine lactones (AHLs) were directly detected in 1-9-day-old subtidal biofilms developed in a coastal fish farm by using AHL reporter strains and gas chromatography-mass spectrometry. Both methods showed that the AHL molecules and/or AHL-producing bacterial community were dynamic during biofilm development, with dominant AHLs changed from short-chain to long-chain AHLs. Terminal restriction fragment length polymorphism analysis of the bacterial 16S rRNA genes derived from subtidal biofilms of different ages was compared to that of the 21 AHL-producing bacteria isolated from the same batch of subtidal biofilms. All terminal restriction fragments (TRFs) generated from AHL-producing bacteria matched with the dominant TRFs derived from the biofilm bacterial community samples. Particularly, the TRFs of all AHL-producing Vibrio spp. matched with the TRFs that were dominant only in 1-day-old biofilm, suggesting that AHL-producing vibrios were one of the pioneer groups during subtidal biofilm formation. We reported here for the first time the dynamics of AHLs and AHL-producing bacteria during the formation of a subtidal biofilm.
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