期刊
MOLECULAR SYSTEMS BIOLOGY
卷 11, 期 7, 页码 -出版社
WILEY
DOI: 10.15252/msb.20145996
关键词
auto-catalysis; cell cycle control; fission yeast Schizosaccharomyces pombe; gradient formation; robustness
资金
- ERC Starting Grant [260493]
- Swiss National Science Foundation research grant [31003A_138177]
- SIB Swiss Institute of Bioinformatics, SystemsX.ch (via PlantMechaniX)
- Swiss National Science Foundation [31003A_130691/1]
- European Research Council (ERC) [260493] Funding Source: European Research Council (ERC)
Concentration gradients provide spatial information for tissue patterning and cell organization, and their robustness under natural fluctuations is an evolutionary advantage. In rod-shaped Schizosaccharomyces pombe cells, the DYRK-family kinase Pom1 gradients control cell division timing and placement. Upon dephosphorylation by a Tea4-phosphatase complex, Pom1 associates with the plasma membrane at cell poles, where it diffuses and detaches upon auto-phosphorylation. Here, we demonstrate that Pom1 auto-phosphorylates intermolecularly, both invitro and invivo, which confers robustness to the gradient. Quantitative imaging reveals this robustness through two system's properties: The Pom1 gradient amplitude is inversely correlated with its decay length and is buffered against fluctuations in Tea4 levels. A theoretical model of Pom1 gradient formation through intermolecular auto-phosphorylation predicts both properties qualitatively and quantitatively. This provides a telling example where gradient robustness through super-linear decay, a principle hypothesized a decade ago, is achieved through autocatalysis. Concentration-dependent autocatalysis may be a widely used simple feedback to buffer biological activities.
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