期刊
MOLECULAR SYSTEMS BIOLOGY
卷 11, 期 12, 页码 -出版社
WILEY-BLACKWELL
DOI: 10.15252/msb.20156639
关键词
immature and mature olfactory sensory neurons; odorant receptor; olfactory system; single-cell RNA-Seq; trace amine-associated receptor (TAAR)
资金
- NIH [R01 EB010244, DP1 CA186693, R01 DC013289]
- HHMI International Student Research Fellowship
In mammals, each olfactory sensory neuron randomly expresses one, and only one, olfactory receptor (OR)a phenomenon called the one-neuron-one-receptor rule. Although extensively studied, this rule was never proven for all similar to 1,000 OR genes in one cell at once, and little is known about its dynamics. Here, we directly tested this rule by single-cell transcriptomic sequencing of 178 cells from the main olfactory epithelium of adult and newborn mice. To our surprise, a subset of cells expressed multiple ORs. Most of these cells were developmentally immature. Our results illustrated how the one-neuron-one-receptor rule may have been established: At first, a single neuron temporarily expressed multiple ORsseemingly violating the ruleand then all but one OR were eliminated. This work provided experimental evidence that epigenetic regulation in the olfactory system selects a single OR by suppressing a few transiently expressed ORs in a single cell during development.
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