期刊
MOLECULAR PLANT PATHOLOGY
卷 16, 期 7, 页码 699-709出版社
WILEY
DOI: 10.1111/mpp.12228
关键词
avirulence gene; Brassica napus; comparative genomics; Leptosphaeria maculans
资金
- Government of Saskatchewan Agricultural Development Fund (ADF)
- Growing Forward 2 (SaskCanola)
- Growing Forward 2 (AAFC)
- Marie Curie Training Site FunGene [HPMT-CT-2001-00395]
- FunGene project
Five avirulence genes from Leptosphaeria maculans, the causal agent of blackleg of canola (Brassica napus), have been identified previously through map-based cloning. In this study, a comparative genomic approach was used to clone the previously mapped AvrLm2. Given the lack of a presence-absence gene polymorphism coincident with the AvrLm2 phenotype, 36 L.maculans isolates were resequenced and analysed for single-nucleotide polymorphisms (SNPs) in predicted small secreted protein-encoding genes present within the map interval. Three SNPs coincident with the AvrLm2 phenotype were identified within LmCys1, previously identified as a putative effector-coding gene. Complementation of a virulent isolate with LmCys1, as the candidate AvrLm2 allele, restored the avirulent phenotype on Rlm2-containing B.napus lines. AvrLm2 encodes a small cysteine-rich protein with low similarity to other proteins in the public databases. Unlike other avirulence genes, AvrLm2 resides in a small GC island within an AT-rich isochore of the genome, and was never found to be deleted completely in virulent isolates.
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