期刊
MOLECULAR PLANT
卷 8, 期 12, 页码 1795-1808出版社
CELL PRESS
DOI: 10.1016/j.molp.2015.10.005
关键词
rare variant; TCP transcription factor; cucumber tendril; homologous organs
资金
- National Natural Science Foundation of China (NSFC) [31225025]
- National Program on Key Basic Research Projects in China (the 973 program) [2012CB113900]
- Chinese Ministry of Finance [1251610601001]
- CAAS Agricultural Science and Technology Innovation Program grant
Rare genetic variants are abundant in genomes but less tractable in genome-wide association study. Here we exploit a strategy of rare variation mapping to discover a gene essential for tendril development in cucumber ( Cucumis sativus L.). In a collection of > 3000 lines, we discovered a unique tendril-less line that forms branches instead of tendrils and, therefore, loses its climbing ability. We hypothesized that this unusual phenotype was caused by a rare variation and subsequently identified the causative single nucleotide polymorphism. The affected gene TEN encodes a TCP transcription factor conserved within the cucurbits and is expressed specifically in tendrils, representing a new organ identity gene. The variation occurs within a protein motif unique to the cucurbits and impairs its function as a transcriptional activator. Analyses of transcriptomes from near-isogenic lines identified downstream genes required for the tendril's capability to sense and climb a support. This study provides an example to explore rare functional variants in plant genomes.
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