期刊
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
卷 56, 期 1, 页码 185-189出版社
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.14-15884
关键词
Boston Keratoprosthesis; penetrating keratoplasty; cornea inflammation; syngeneic; allogeneic
资金
- Boston-KPro research fund (Boston, MA, USA)
- National Institutes of Health (Bethesda, MD, USA) [R01-EY 12963]
- NATIONAL EYE INSTITUTE [R01EY012963] Funding Source: NIH RePORTER
PURPOSE. To compare corneal inflammation after syngeneic and allogeneic penetrating keratoplasty (PK) with miniature Keratoprosthesis (m-KPro) implantation in mice. METHODS. BALB/C (syngeneic) or C57BL/6 (allogeneic) corneas were transplanted onto BALB/C host beds as part of PK or m-KPro implantation. Corneal inflammation was assessed by determining the frequencies of CD45(+) leukocytes, CD4(+) T cells, CD11b(+) cells, and Gr-1(+) granulocytes/monocytes by flow cytometry at 2, 4, and 8 weeks post transplantation. In addition, expression levels of the proinflammatory cytokines TNF-alpha and IL-1 beta were analyzed using real-time qPCR at 8 weeks post transplantation. RESULTS. Cell frequencies in the syngeneic (syn) and allogeneic (allo) m-KPro groups were higher compared with the syngeneic and allogeneic PK groups, respectively, at all time points. However, after week 4, frequencies of all analyzed immune cells were higher in the alloPK group as compared with synKPro group. At 8 weeks, the expression of TNF-alpha was higher in synKPro, alloPK, and alloKPro groups compared with the naive and synPK groups. The expression of IL-1 beta was significantly higher in both KPro groups as compared with PK groups. CONCLUSIONS. Although the m-KPro device augments the inflammatory response in the cornea after its implantation, allogenicity (of the carrier tissue) is also a significant contributor to corneal inflammation. These data suggest that using syngeneic or decellularized corneal tissue as a Boston-KPro carrier could reduce the postoperative inflammation response.
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