Transcripts of the nicotinic acetylcholine receptor subunit gene Pxylα6 with premature stop codons are associated with spinosad resistance in diamondback moth, Plutella xylostella

The cDNA sequence of the alpha 6 nicotinic acetylcholine receptor subunit of diamondback moth (Plutella xylostella) was cloned and sequenced. Transcripts were similar between the spinosad-susceptible G88 and Wapio strains. All transcripts from the spinosad-resistant Pearl-Sel strain contained premature stop codons, and most transcripts have not been previously reported. None of these truncated transcripts were seen in the spinosad-susceptible strains. Proteins made from these transcripts would likely have no, or greatly altered, receptor function. An F-2 backcross and spinosad bioassay showed that all spinosad bioassay survivors produced truncated alpha 6 transcripts. Thus, it appears that spinosad resistance in diamondback moth is due to a mutation(s) that results in no functional Pxyl alpha 6 being produced.