期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 59, 期 6, 页码 1076-1087出版社
WILEY-BLACKWELL
DOI: 10.1002/mnfr.201500005
关键词
Deoxynivalenol; Goblet cell; Mucin; Mucus; Mycotoxin
资金
- Centre National de la Recherche Scientifique (CNRS)
- Institut National de la Recherche Agronomique (INRA)
- Ministere de l'Enseignement Superieur et de la Recherche Scientifique
- ANR grant ImBIO
ScopeThe food-associated mycotoxin deoxynivalenol (DON) is known to affect intestinal functions. However, its effect on intestinal mucus is poorly characterized. Methods and resultsWe analyzed the effects of DON on human goblet cells (HT29-16E cells) and porcine intestinal explants. Results showed that subtoxic doses of DON (as low as 1 M) decreased mucin (MUC) production. qPCR analysis demonstrated that this inhibition was due to a specific decrease in the level of mRNA encoding for the intestinal membrane-associated (MUC1) and the secreted MUCs (MUC2, MUC3). Mechanistic studies demonstrated that DON effect relied on the activation of the protein kinase R and the mitogen-activated protein kinase p38 ultimately leading to the inhibition of the expression of resistin-like molecule beta, a known positive regulator of MUC expression. ConclusionTaken together, our results show that at low doses found in food and feed, DON is able to affect the expression and production of MUCs by human and animal goblet cells. Due to the important role of MUCs in the barrier function and in the interaction of commensal bacteria with the host, such effect could explain the observed modifications in the microbial diversity and the increased susceptibility to enteric infection following exposure to DON.
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