期刊
INTERNATIONAL JOURNAL OF UROLOGY
卷 16, 期 7, 页码 639-646出版社
WILEY
DOI: 10.1111/j.1442-2042.2009.02325.x
关键词
acrosome; retrotransposition; salivary gland; sperm; spermatogenesis; spermiogenesis; testis; X chromosome
Objective: To examine the expression profiles of the proteins translated from Acpin1 mRNA in germ cells. Methods: Northern and western blotting of various tissues and immunohistochemical analysis of germ cells were carried out in a mouse model. Results: ACPIN1 protein was transcribed from the longer, 3' open reading frame (ORF) of Acpin1. An alternative-splicing variant, Acpin1vs, contained only the smaller, 5' ORF of the full-length Acpin1 gene. Its gene product, SAGSIN1, was expressed specifically in salivary glands. Retrotransposed regions of Acpin1 homology were also detected in various chromosomes, and intronless paralogous genes on the X chromosome were expressed in the testis and other tissues. The genomic structure of Acpin1 is highly conserved in mammals. Conclusion: The two ORFs on the Acpin1 mRNA are independently translated in differentiated cells. Analysis of gene Acpin1 might clarify the molecular mechanism of spermatogenesis.
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