期刊
MOLECULAR MICROBIOLOGY
卷 97, 期 1, 页码 166-178出版社
WILEY
DOI: 10.1111/mmi.13018
关键词
-
资金
- NIH [AI064184, AI076322, GM103655]
- Welch Foundation [F-1155]
- Army Research Office [W911NF-12-1-0390]
- Cystic Fibrosis Foundation [Trent13G0]
Gram-negative bacteria survive harmful environmental stressors by modifying their outer membrane. Much of this protection is afforded upon remodeling of the lipid A region of the major surface molecule lipopolysaccharide (LPS). For example, the addition of cationic substituents, such as 4-amino-4-deoxy-L-arabinose (L-Ara4N) and phosphoehthanolamine (pEtN) at the lipid A phosphate groups, is often induced in response to specific environmental flux stabilizing the outer membrane. The work herein represents the first report of pEtN addition to Pseudomonas aeruginosa lipid A. We have identified the key pEtN transferase which we named EptA(Pa) and characterized its strict activity on only one position of lipid A, contrasting from previously studied EptA enzymes. We further show that transcription of eptA(Pa) is regulated by zinc via the ColRS two-component system instead of the PmrAB system responsible for eptA regulation in E.coli and Salmonella enterica. Further, although L-Ara4N is readily added to the same position of lipid A as pEtN under certain environmental conditions, ColR specifically induces pEtN addition to lipid A in lieu of L-Ara4N when Zn2+ is present. The unique, specific regulation of eptA(Pa) transcription and enzymatic activity described in this work demonstrates the tight yet inducible control over LPS modification in P.aeruginosa.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据