期刊
MOLECULAR MEDICINE REPORTS
卷 13, 期 1, 页码 994-1002出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2015.4632
关键词
microRNA-362-3p; tumor suppressor; renal cancer cells; nemo-like kinase
资金
- National Natural Science Foundation of China [81170613, 81270740]
- Shenzhen Basic Research knowledge Innovation Program [JCYJ20140416180 323426]
MicroRNAs (miRNAs) have been demonstrated to exhibit abnormal expression patterns in various types of human cancer. The aim of the present study was to identify a novel tumor suppressor microRNA (miR) and investigate its physiological function and mechanism in renal cell carcinoma (RCC). The expression levels of miRNA (miR)-362-3p expres were measured in 47 pairs of RCC and adjacent normal tissue samples, using reverse transcription-quantitative polymerase chain reaction analysis. In addition, miR-362-3p was transfected into renal cancer cells to investigate its role in the regulation of cell proliferation, migration, invasion, apoptosis and cell cycle. Identification of the target gene of miR-362-3p was performed using luciferase reporter assays and western blot analyses. The results demonstrated that the expression levels of miR-362-3p were downregulated in the RCC tissue samples, compared with the adjacent normal tissue samples. The upregulation of miR-362-3p using a synthesized mimic suppressed the proliferation, migration and invasion of the renal cancer cells, and induced cell apoptosis and G(1) phase arrest. Further experiments demonstrated that the overexpression of miR-362-3p resulted in decrease expression levels of nemo-like kinase. These results suggested that miR-362-3p functions as a tumor suppressor in RCC, and may serve as a potential molecular target in the treatment of RCC.
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