期刊
MOLECULAR MEDICINE REPORTS
卷 11, 期 6, 页码 4365-4372出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2015.3273
关键词
microRNA; human testicle cells; embryonic carcinoma of testis; male infertility
资金
- Science and Technology Department of Sichuan Province, China
MicroRNAs (miRs) have crucial functions in spermatogenesis and implications for male infertility. In the present study, Homo sapiens (hsa)-miR-145 was designed and cloned into the eukaryotic expression plasmid pGenesil-1. The recombinant plasmids were transfected into Hs 1.tes normal testicular cells and NTERA-2 testicular cancer cells. Quantitative polymerase chain reaction of hsa-miR-145 indicated that pGenesil-1-miR-145 effectively upregulated. the expression of hsa-miR-145 in vitro. hsa-miR-145 overexpression inhibited the mRNA and protein expression of sex-determining region Y Box 9 in Hs 1.tes cells. The proliferation rates of NTERA-2 cells transfected with pGenesil-1-miR-145 were significantly decreased. High expression levels of miR-145 promoted cell apoptosis in NTERA-2 cells. The results revealed that altered hsa-miR-145 expression in testicular cells affects the regulation of target genes associated with male infertility.
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