期刊
MOLECULAR MEDICINE REPORTS
卷 12, 期 5, 页码 7389-7395出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2015.4390
关键词
castration-resistant prostate cancer; proliferation; p21-activated protein kinase 6; microRNA-328; docetaxel
资金
- National Natural Science Foundation of China [81370849, 81300472, 81202034]
- Natural Science Foundation of Jiangsu Province [BL2013032, BK2012336]
- Nanjing City [201201053]
- Southeast University [3290002402]
- Science Foundation of Ministry of Education of China [20120092120071]
- Fundamental Research Funds for the Central Universities
- Scientific Research Innovation Project of University in Jiangsu Province [KYLX_0203]
Prostate cancer (Pca) has one of the highest mortality rates for malignant cancers worldwide. Previous research has demonstrated that numerous genes are aberrantly expressed during Pea onset and development, p21-activated protein kinase 6 (PAK6) is known to be overexpressed in primary and metastatic Pea, however the mechanism of this aberrant expression remains unknown. In the present study, immunohistochemistry demonstrated that PAK6 is overexpressed in castration-resistant Pea (CRPC). Furthermore, PAK6 overexpression was regulated by microRNA (miR)-328. Luciferase reporter assay and western blot analysis indicated that PAK6 was directly targeted by miR-328. Forced expression of miR-328 enhanced docetaxel sensitivity, inhibited cell proliferation and promoted cell apoptosis without affecting the cell cycle. This indicates that miR-328 performs important functions in CRPC progression via PAK6 regulation. This mechanism may be used to enhance the effect of docetaxel.
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