期刊
MOLECULAR MEDICINE REPORTS
卷 12, 期 2, 页码 2701-2706出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2015.3724
关键词
microRNA-18a; estrogen receptor alpha; JEG-3 cell; invasion; apoptosis
资金
- Chinese Natural Science Foundation [81471474/31000660, 81170582]
- Postdoctoral Science Foundation of China [2014T70964, 2013M532203]
- Tangdu Hospital Reserve Personnel Fund
The purpose of the present study was to gain further understanding of the function of microRNA-18a (miR-18a) expression in the JEG-3 human trophoblast cell line. JEG-3 cells were transfected with pre-miR-18a mimics or miR-18a inhibitors. The effects of miR-18a on trophoblast cell invasion and apoptosis, and on the expression of estrogen receptor alpha (ESR alpha) were analyzed using a Transwell invasion assay, flow cytometry, reverse transcription-polymerase chain reaction, western blot analysis and a luciferase assay. The results of the present study suggested that miR-18a expression suppression led to a decrease in JEG-3 cell invasion and an increase in JEG-3 cell apoptosis, by inducing ESR alpha expression. The present study provides evidence for the involvement of miR-18a in the pathogenesis of pre-eclamptic pregnancies.
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