期刊
MOLECULAR MEDICINE REPORTS
卷 13, 期 2, 页码 1179-1185出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2015.4662
关键词
CRMP-5; actin; growth cone; hippocampal neuron; neurite growth
资金
- National Natural Science Foundation of China [31170941, 31300885]
- Natural Science Foundation of Guangdong Province, China [S20130100141191, 2014A030310024, 2014A030313357]
- Medical Scientific Research Foundation of Guangdong Province, China [A2014382, A2015479]
- Fundamental Research Funds for the Central Universities [21615477]
CRMP family proteins (CRMPs) are abundantly expressed in the developing nervous system mediating growth cone guidance, neuronal polarity and axon elongation. CRMP-5 has been indicated to serve a critical role in neurite outgrowth. However, the detailed mechanisms of how CRMP-5 regulates neurite outgrowth remain unclear. In the current study, co-immunoprecipitation was used to identify the fact that CRMP-5 interacted with the actin and tubulin cytoskeleton networks in the growth cones of developing hippocampal neurons. CRMP-5 exhibited increased affinity towards actin when compared with microtubules. Immunocytochemistry was used to identify the fact that CRMP-5 colocalized with actin predominantly in the C-domain and T-zone in growth cones. In addition, genetic inhibition of CRMP-5 by siRNA suppressed the expression of actin, growth cone development and neurite outgrowth. Overexpression of CRMP-5 promoted the interaction with actin, growth cone development and hippocampal neurite outgrowth. Taken together, these data suggest that CRMP-5 is able to interact with the actin cytoskeleton network in the growth cone and affect growth cone development and neurite outgrowth via this interaction in developing hippocampal neurons.
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