期刊
MOLECULAR MEDICINE REPORTS
卷 12, 期 3, 页码 4759-4765出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2015.3996
关键词
naringin; bone marrow stem cells; peroxisome proliferator-activated receptor gamma; microRNA-20a
Naringin is a dihydrotestosterone flavonoid compound that significantly inhibits bone loss, improves bone density, and enhances biomechanical anti-compression performance. Previous studies have demonstrated that naringin improves the activity levels of osteocalcin (OC) and alkaline phosphatase (ALP) in MC3T3-E1 osteoblast precursor cells. The present study investigated the effects of naringin on osteoblastic differentiation and inhibition of adipocyte formation in bone marrow stem cells (BMSCs). The levels of osteogenesis were modulated via upregulation of the expression levels of microRNA (miR)-20a, and downregulation of the expression levels of peroxisome proliferator-activated receptor. (PPAR.). The results indicated that naringin significantly enhanced BMSC proliferation in a dose-dependent manner. In addition, naringin significantly increased the mRNA expression levels of OC, ALP, and collagen type I. Furthermore, naringin decreased the protein expression levels of PPAR., and increased the expression levels of miR-20a in the BMSCs. These results suggested that miR-20a may regulate the expression of PPAR. in BMSCs. To our knowledge, this is the first study to report naringin-induced osteogenesis via upregulation of the expression levels of miR-20a, and downregulation of the expression levels of PPAR gamma. These results indicated the important role of naringin in BMSC differentiation.
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