Lactobacillus porci sp nov., isolated from small intestine of a swine

A facultatively anaerobic, Gram-stain-positive, catalase-negative, rod-shaped lactic acid bacterium, designated SG816(T), was isolated from small intestine of a swine. Optimal growth occurred at 37 degrees C and pH 7.0. Furthermore, growth occurred in NaCl up to 0.5 % (w/v) but not at levels of salinity higher than 1 %. Comparative 16S rRNA gene sequencing and the matrix-associated laser desorption/ionization-time-of-flight mass spectometry profiling showed that strain SG816(T) was closely related to Lactobacillus delbrueckiisubsp. bulgaricus KCTC 3635(T) (95.9 %) and Lactobacillus delbrueckiisubsp. indicus JCM 15610(T) (95.9 %), followed by other Lactobacillus delbrueckii subspecies (95.9-95.7%) and Lactobacillus equicursoris DSM 19284(T) (95.6 %). A comparison of two housekeeping genes, RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS), revealed that strain SG816(T) formed a separate branch within the Glade of the genus Lactobacillus. The DNA G+C content level of the strain SG816(T) was 51.5 mol%. The strain was homofermentative and produced D-lactic acid from glucose fermentation. The major cellular fatty acids (>10 %) of the isolate were C-18:0 omega 9c and C-16:0. The peptidoglycan type was A4 alpha L-Lys-D-Asp. On the basis of distinct phenotypic and phylogenetic properties, strain SG816(T) represents a novel species of the genus Lactobacillus, for which the name Lactobacillus porci sp. nov. is proposed. The type strain is SG816(T )(=KCTC 21090(T)=NBRC 112917(T)).