Tahibacter caeni sp nov., isolated from activated sludge

A Gram-reaction-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated BUT-6(T), was isolated from activated sludge of a wastewater-treatment facility. The strain grew at 15-35 degrees C (optimum 30 degrees C), pH 4.0-10.0 (optimum pH 7.0) and 0-3.0% (w/v) NaCl (optimum 1.0%). Phylogenetic analysis based on 16S rRNA sequences showed that strain BUT-6(T) was most closely related to Tahibacter aquaticus PYM5-11(T) (98.6 % similarity). However, the DNA DNA relatedness between strain BUT-6(T) and T. aquaticus PYM5-11(T) was 47.1 %. The major fatty acids (>10% of total fatty acids) of strain BUT-6(T) were iso-C-15:0, iso-C-17:1 omega 9c and iso-C-17:0. The major respiratory quinone was ubiquinone O-8. The profile of polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminophospholipid, three unknown aminolipids and unidentified phospholipids. The DNA G+C content of strain BUT-6(T) was 71.7 mol%. On the basis of the data from the polyphasic taxonomic study presented, strain BUT-6(T) is considered to represent a novel species of the genus Tahibacter, for which the name Tahibacter caeni sp. nov. is proposed. The type strain is BUT-6(T) (=CCTCC AB 2013266(T)=KACC 17139(T)).