Ramlibacter solisilvae sp nov., isolated from forest soil, and emended description of the genus Ramlibacter

A Gram-staining-negative, strictly aerobic, white-colony-forming bacterium, designated strain 5-10(T), was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods or coccoids, showing oxidase(-) and catalase-positive reactions. Growth was observed at 10-37 degrees C (optimum, 30 degrees C), at pH 5.0-9.0 (optimum, pH 7.0) and in the presence of 0-1.0% (w/v) NaCl (optimum, 0-0.5 %). The major cellular fatty acids were summed feature 3 (comprising C-16:1 omega 6c and/or C-16:1 omega 7c), C-16:0, C-10:0 3-OH and summed feature 8 (comprising C-18:1 omega 6c and/or C-18:1 omega 7c). The G+C content of the genomic DNA was 69.9 mol% and the only respiratory quinone detected was ubiquinone 8 (Q-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 5-10(T) formed a tight phyletic lineage with members of the genus Ramlibacter. Strain 5-10(T) was most closely related to Ramlibacter tataouinensis TTB310(T) (97.3%), but the DNA-DNA relatedness level between the two strains was 38.2 +/- 1.8%. Based on phenotypic, chemotaxonomic and molecular features, strain 5-10T was shown to represent a novel species of the genus Ramlibacter, for which the name Ramlibacter solisilvae sp. nov. is proposed. The type strain is 5-10(T) (=KACC 17567(T)=JCM 19319(T)). An emended description of the genus Ramlibacter is also proposed.