Mumia flava gen. nov., sp nov., an actinobacterium of the family Nocardioidaceae

A novel actinobacterial strain, designated MUSC 201(T), was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201(T) represented a novel lineage within the class Actinobacteria. Strain MUSC 201(T) formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9-95.1%), Aeromicrobium (92.7-94.6%), Marmoricola (92.5-93.1 %) and Kribbella (91.5-92.4%). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained LL-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3 gamma. The peptidoglycan cell wall contained LL-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5 :0.9 :1.0 :1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H-4). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids Were C-18:1 omega 9c(30.8 %), C-16:0 (24.1 7), and 10-methyl C-18:0 (13.0 %). The DNA G+C content was 72.0 +/- 0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae, a novel genus and species, Mumia [lava gen. nov., sp. nov. are proposed. The type strain of Mumia flava is MUSC 201(T) (=DSM 27763(T)=MCCC 1A00646(T)=NBRC 109973(T)).