Halomonas huangheensis sp nov., a moderately halophilic bacterium isolated from a saline-alkali soil

A novel, Gram-stain-negative, aerobic, rod-shaped, non-motile and moderately halophilic bacterium, designated strain BJGMM-B45(T), was isolated from a saline-alkali soil collected from Shandong Province, China. Growth of strain BJGMM-B45(T) occurred at 10-45 degrees C (optimum, 30 degrees C) and pH 5.0-12.0 (optimum, pH 7.0) on Luria-Bertani agar medium with 1-20% (w/v) NaCl (optimum, 7-10%). The predominant respiratory quinone was Q-9. The major cellular fatty acids (>5%) were C-18:1 omega 7c, C-16:0, C-19:0 cyclo omega 8c, summed feature 3, C-12:0 3-OH and C-12:0. The genomic DNA G+C content was 57.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BJGMM-B45(T) belonged to the genus Halomonas in the class Gammaproteobacteria. The closest relatives were Halomonas cupida DSM 4740(T) (98.2 % 16S rRNA gene sequence similarity) and Halomonas denitrificans M29(T) (97.8 %). Levels of DNA-DNA relatedness between strain BJGMM-B45(T) and Halomonas cupida CGMCC 1.2312(T) and Halomonas denitrificans DSM 18045(T) were 57.0 and 58.9 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BJGMM-B45(T) is considered to represent a novel species of the genus Halomonas, for which the name Halomonas huangheensis sp. nov. is proposed. The type strain is BJGMM-B45(T) (=ACCC 05850(T)=KCTC 32409(T)).