Nocardioides szechwanensis sp nov and Nocardioides psychrotolerans sp nov., isolated from a glacier

Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT1-17(T) and RHLT2-1(T)) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Nocardioides and shared 97.8% sequence similarity with each other and 97.6 and 98.4% sequence similarity, respectively, with Nocardioides kribbensis KSL-2(T). Strain RHLT1-17(T) grew at 0-35 degrees C and strain RHLT2-1(T) grew at 0-25 degrees C. The major cellular fatty acids of strain RHLT1-17(T) were C-17:1 omega 8c (32.69%) and iso-C-16:0 (21.74 %). The major cellular fatty acids of strain RHLT2-1(T) were C-18:1 omega 9c (28.72 %), summed feature 3 (17.14%; comprising C-16:1 omega 7c and/or C-16:1 omega 6c), iso-C-16:0 (14.35%), C-16:0 (9.96%) and iso-C-14:0 (8.34%). Both strains contained LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H-4) as the predominant menaquinone. On the basis of data obtained using a polyphasic approach, two novel species, Nocardioides szechwanensis sp. nov. (type strain RHLT1-17(T) =CGMCC 1.11147(T)=NBRC 108562(T)) and Nocardioides psychrotolerans sp. nov. (type strain RHLT2-1(T) =CGMCC 1.11156(T)=NBRC 108563(T)), are proposed.