Nocardioides marinquilinus sp nov., isolated from coastal seawater

A Gram-stain-positive, non-motile, strictly aerobic, non-spore-forming and short rod-shaped bacterial strain, CL-GY44(T), was isolated from coastal seawater, Korea. Analysis of the 16S rRNA gene sequence of strain CL-GY44(T) revealed a clear affiliation with the genus Nocardioides. Based on phylogenetic analysis, strain CL-GY44(T) showed the closest phylogenetic relationship with Nocardioides ginsengagri BX5-10(T) and Nocardioides plantarum NCIMB 12834(T). Strain CL-GY44(T) was not able to grow in the presence of NaCl but grew with 0-5.5 % sea salts. The optimum temperature and pH for growth were 30 C and pH 7.0. The major cellular fatty acids of strain CL-GY44(T) were C-17:1 omega 6c, iso-C-16:0, C-16:0 and iso-C-15:0 and the major menaquinone was MK-8(H-4). The cell-wall analysis showed that strain CL-GY44(T) contained LL-diaminopimelic acid. The genomic DNA G+C content was 71.6 molok. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-GY44(T) could be clearly distinguished from members of the genus Nocardioides. Thus, strain CL-GY44(T) should be classified as representing a novel species in the genus Nocardioides, for which the name Nocardioides marinquilinus sp. nov. is proposed. The type strain is CL-GY44(T) (=KCCM 90109(T)=JCM 18459(T)).