Micromonospora sediminicola sp nov., isolated from marine sediment

An actinomycete strain, designated strain SH2-13(T), was isolated from a marine sediment sample collected from the Andaman Sea of Thailand. Applying a polyphasic approach, the isolate was identified as a member of the genus Micromonospora using morphological and chemotaxonomic characteristics, including the presence of meso-diaminopimelic acid in the peptidoglycan. Whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. Diagnostic polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and phosphoglycolipids. The major menaquinones were MK-10(H-2), MK-10(H-4) and MK-10(H-6). 16S rRNA gene sequence analysis revealed similarity to Micromonospora marina JSM1-1(T) (99.1%), Micromonospora coxensis 2-30-b(28)(T) (99.1 %), Micromonospora aurantiaca DSM 43813(T) (98.8%) and Micromonospora chalcea DSM 43026(T) (98.7%). However, a combination of DNA DNA hybridization results and phenotypic properties indicated that strain SH2-13(T) (=NBRC 107934(T)=BCC 45601(T)) should be classified as the type strain of a novel species, with the proposed name Micromonospora sediminicola sp. nov.