Knoellia flava sp nov., isolated from pig manure

A Gram-positive, aerobic, non-spore-forming actinobacterial strain, designated strain TL1(T), was isolated from pig manure in Wuhan, China. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol. The predominant menaquinone was MK-8(H-4). The major fatty acids (>10%) were iso-C-16:0, iso-C-15:0 and C-17:1 omega 8C. The genomic DNA G+C content was 70.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TL1(T) was most closely related to the type strains of Knoellia sinensis (98.5%), Knoellia subterranea (98.2%) and Knoellia aerolata (96.9 %). DNA DNA relatedness values of strain TL1(T) with the type strains of K. sinensis and K. subterranea were 27.3 and 34.0%, respectively. Comparison of phenotypic, chemotaxonomic and phylogenetic characteristics among strain TL1(T) and related organisms revealed that the isolate represents a novel species of the genus Knoellia, for which the name Knoellia flava sp. nov. is proposed; the type strain is TL1(T) (=CGMCC 1.10749(T)=KCTC 19810(T)).